TRIzol Reagent

RNA isolation / purification Tissue - Human Kidney

Experiment
RNA isolation / purification Tissue - Human Kidney
Product
TRIzol Reagent from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

Total RNA was also extracted from mouse kidneys and HEK-293 cell lysates using TRIzol® and the RNAeasy Mini Kit (QIAGEN), respectively. hsa-miR-422a was quantified with TaqMan Micro-RNA Assay (Applied Biosystems) using RNU48 as reference gene. mmu-miR-378 (mmu-miR-378a-3p), which is the mouse paralog of hsa-miR-422a, was quantified with TaqMan Micro-RNA Assay (Applied Biosystems) using U6 snRNA for normalization. For mRNA quantification, reverse transcription was performed with the PrimeScript 1st strand cDNA Synthesis Kit (TAKARA). Human and mouse KLK4 was measured with TaqMan Assay (Applied Biosystems) using GAPDH as reference gene. CT values were normalized to endogenous control and the fold-change relative to a control sample (2ΔΔCT) was used for relative expression analysis

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Discussion

Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

5 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Human Kidney using TRIzol Reagent from Thermo Fisher Scientific.

Paper title
Micro-RNA analysis of renal biopsies in human lupus nephritis demonstrates up-regulated miR-422a driving reduction of kallikrein-related peptidase 4.
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

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Videos

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